张先成;张云湖;赵晓宇;李晶;
ZHANG Xian-CHENG,ZHANG Yun-hu,ZHAO XIAO-yu,LI Jing(Institute of Microbiology of Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010,China)

• 1:黑龙江省科学院微生物研究所

摘要(Abstract)：

对枯草芽孢杆菌B29菌株诱导黄瓜根系相关防御反应酶活性的变化趋势进行了研究,结果表明该菌株接种能够诱导黄瓜根系苯丙氨酸解胺酶(PAL)、多酚氧化酶(PPO)和过氧化物酶(POD)活性有不同程度的提高,尤其在挑战接种病原菌后,3种防御反应酶活性上升的幅度比单独接种要高,说明挑战接种有利于诱导抗性的表达。结合枯草芽孢杆菌B29菌株对黄瓜枯萎病的田间防效试验,可以说明诱导抗性是其生防作用机制之一。
The changes of defense enzymes induced by Bacillus subtilis B29 in cucumber roots were studied.Results showed that the activities of defense enzymes(PAL,PPO,POD)were increased to some extent after inoculation with Bacillus subtilis B29.Especially after challenging inoculation with Fusarium oxsporum,the changes of defense enzymes were increased much more than single inoculation.It suggested that challenging inoculation was good for induced systemic resistance.It could come to a conclusion that induced systemic resistance was one of biocontrol mechanisms combined with the control efficiencies of Bacillus subtilis B29 to cucumber Fusarium wilt disease.

关键词(KeyWords)： 枯草芽孢杆菌;诱导抗性;防御酶
Bacillus subtilis;induced systemic resistance;defense enzymes

Abstract:

Keywords:

基金项目(Foundation): 国家农业科技成果转化资金项目(05EFN212300089);; 黑龙江省自然科学基金项目(C200522)

作者(Authors): 张先成;张云湖;赵晓宇;李晶;
ZHANG Xian-CHENG,ZHANG Yun-hu,ZHAO XIAO-yu,LI Jing(Institute of Microbiology of Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010,China)

DOI: 10.15914/j.cnki.wykx.2009.00.014

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