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用TRIZOL法、CTAB法和改良的热硼酸盐法从水稻叶片中提取总RNA,结果表明用这三种方法提取总RNA的OD260nm/OD280nm值分别为1.896、1.992和1.657。琼脂糖电泳表明,用改良的热硼酸盐法提取的RNA降解严重,用TRIZOL法提取的总RNA有部分降解,而CTAB法提取的总RNA完整、未降解,可用于后续实验。因此CTAB法更适于从水稻叶片中提取总RNA。
Abstract：Total RNA from rice leaves was extracted by TRIZOL,CTAB and improved hot-borate methods.The results showed that the D260nm/D280nm values of total RNA by these methods were 1.886,1.992 and 1.657 respectively.After gelose electrophoresis,the RNA extracted by improved hot-borate method was destroyed heavily,and the RNA extracted by TRIZOL method was better.The RNA extracted by CTAB was very good,which meant the extracted RNA was integrated enough to be used in the following experiments.The method of CTAB is more suitable to extract total RNA from the rice leaves.
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基本信息:
DOI：10.15914/j.cnki.wykx.2009.00.001
中图分类号:Q78
引用信息:
[1]林玲,杨燕凌,何海福等.总RNA提取方法的比较与分析[J].武夷科学,2009,25(00):97-100.DOI:10.15914/j.cnki.wykx.2009.00.001.
基金信息:
福建省自然科学基金项目(2007J0243,X0750005);; 福建省教育厅科技计划项目(JA07059)