武夷科学

为了解不同种源木姜叶柯叶片和枝条中甜味剂含量及其与地理环境的关系，采用超声波提取法和比色法，对木姜叶柯根皮苷和三叶苷含量进行测定和比较。结果表明，木姜叶柯不同种源叶片与枝条中根皮苷和三叶苷含量存在显著差异(P<0.05)。江西安福种源叶片中的根皮苷和三叶苷含量均最多，分别为0.623和0.450μg·g^-1,但枝条中根皮苷含量最少，仅0.103μg·g^-1;福建顺昌种源枝条中的根皮苷含量最高，达0.316μg·g^-1;湖南芷江种源枝条中三叶苷含量最高，为0.180μg·g^-1,是重庆梁平种源的1.98倍。从甜味剂含量与地理气候因子的相关性分析发现，叶片和枝条中的根皮苷和三叶苷含量与年平均相对湿度呈一定相关性，其中叶片中根皮苷和三叶苷含量与年平均相对湿度的相关系数分别为-0.667、-0.812,表明高湿环境不利于叶片成分含量的积累。综上，不同种源木姜叶柯叶片和枝条中甜味剂含量存在明显差异，其中江西安福的叶片和福建顺昌的枝条中的根皮苷和三叶苷含量较高，且经纬度、极端气温、年平均相对湿度等地理气候因子对其成分含量变化有显著影响。

The content of sweet components in the leaves and branches of Lithocarpus litseifolius from different provenances was determined and their relationship with the geographical environment was explored. The ultrasonic extraction method and colorimetric method were adopted to measure and compare the content of phlorizin and trilobatin in L. litseifolius. The results showed differences(P<0.05) in the content of phlorizin and trilobatin in the leaves and branches of L. litseifolius from different provenances. The L. litseifolius sample from Anfu, Jiangxi had the highest content(0.623 and 0.450 μg·g^-1, respectively) of phlorizin and trilobatin in the leaves, while its content of phlorizin in the branches was the lowest, only 0.103 μg·g^-1. The phlorizin content in the branches of the sample from Shunchang, Fujian was the highest, reaching 0.316 μg·g^-1. The content of trilobatin in the branches of the sample from Zhijiang, Hunan was the highest, which was 0.180 μg·g^-1 and 1.98 times that of the sample from Liangping, Chongqing. The correlation analysis between sweet components and geographical and climatic factors showed that the content of phlorizin and trilobatin in the leaves and branches had a correlation with the annual average relative humidity. The correlation coefficients between the content of phlorizin and trilobatin in the leaves and the annual average relative humidity were-0.667 and-0.812, respectively, indicating that a highly humid environment was not conducive to the accumulation of components in the leaves. In summary, the content of phlorizin and trilobatin in the leaves and branches demonstrates obvious differences among L. litseifolius from different provenances. The content of phlorizin and trilobatin in the leaves of L. litseifolius from Anfu, Jiangxi and in the branches of L. litseifolius from Shunchang, Fujian are high. Moreover, geographical and climatic factors such as longitude, latitude, extreme temperatures, and annual average relative humidity have significant effects on the content of the components.